Abstract
The objective of this study was to correlate the Biuret, Lowry, Bradford, and Mlikoscan methods used to determine the level of protein in goat's milk and compare them with the Kjeldahl method. Milk samples collected from 18 goats of breeds Saanen (n=5), Anglo-Nubian (n=4), Pardo-alpina (n=5), and Creole (n=4) were analyzed. Correlation analyses between the different methods were performed using the Pearson test and the protein content between different breeds was compared using ANOVA. Total protein (TP) and casein (CN) concentrations obtained by all methods studied were positively correlated with the Kjeldahl method (p<0.01). The Bradford method presented the lowest correlation coefficient. All methods apart from Bradford showed significant differences in TP and CN between the Anglo-Nubian and Pardo-alpina breeds (p<0.05). The Bradford method showed significant differences in TP between the Saanen and Pardo-alpina breeds (p<0.05), and no differences were found between breeds reegarding CN. In conclusion, the Bradford method had the lowest correlation value (TP and CN) with the Kjeldahl, and the Bradford method did not show the same pattern of differences between breeds in TP and CN, as was evidenced in all other methods. Therefore, the use of the Bradford method is not recommended to determine proteins in goat’s milk.
Highlights
RESUMO O objetivo deste estudo foi correlacionar os métodos de Biuret, Lowry, Bradford e Mlikoscan utilizados para a determinação da proteína do leite de cabra e compará-los com o método de Kjeldahl
Milk proteins play an important role in technological products, such as the production of artisanal cheeses, which is of great importance in goat rearing
Considering this information, the objective of this study was to correlate different methods used in the determination of proteins in goat's milk (Lowry, Biuret, Bradford and Mlikoscan) and contrast them with the standard method of Kjeldahl
Summary
RESUMO O objetivo deste estudo foi correlacionar os métodos de Biuret, Lowry, Bradford e Mlikoscan utilizados para a determinação da proteína do leite de cabra e compará-los com o método de Kjeldahl. Different studies use different methods to determine the protein content in goat's milk (Biuret: RAIMONDO et al 2013, Bradford: DOS SANTOS et al 2017, Kjeldahl: OLALLA et al 2009, Lowry: AL-ABDULKARIM et al 2013, Milkoscan: EL-TARABANY et al 2018); with the Kjeldahl method as a reference (BRADLEY et al 1992, ZENG 1996). Damián & Sacchi studies that evaluated and correlated different methods to determine protein content in milk, they were made with human milk (KELLER & NEVILLE 1986, LONNERDAL et al 1987).
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