Abstract

Determination of Phytochemical Constituent, Antioxidant Activity, Total Phenol and Total Flavonoid of Extract Ethanol Phyllanthus emblica Fruit

Highlights

  • Phyllanthus emblica (PE) is a plant that grows widely in Indonesia, on Sumatra island

  • Phyllanthus emblica (PE) is a plant that is regularly found in Indonesia, on Sumatra island, under the name Balakka

  • The phytochemical analysis of Phyllanthus emblica ethanol extract was performed using the TSQ Exactive (Thermo) gradient technique (LSIH, Brawijaya University) with mobile phase A (0.1 percent formic acid in water) and phase B. (0.1 percent formic acid in acetonitrile)

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Summary

Introduction

Phyllanthus emblica (PE) is a plant that grows widely in Indonesia, on Sumatra island. PE fruit is well-known for its high antioxidant activity and a variety of pharmacological properties. The solution was filtered using Whatman paper no 1, and the filtered result was evaporated under reduced pressure using a rotary evaporator until a crude extract/ethanol extract of PE (EEPE) was obtained, and the phytochemical constituents, antioxidant activity, total phenol, and flavonoid were analysed. EEPE as an antioxidant of 7.626 ± 0.41 μg/dL It shows that the antioxidant activity of the ethanol extract of Phyllanthus emblica is strong ethanol extract of Phyllanthus emblica contains Total Flavonoid was 5.816 ± 2.81(mg QE/g extract) and total phenol was 274.590 ± 13.61(mg GAE/g extract). Conclusions: In summary, extract ethanol of Pyllanthus emblica contains flavonoid and have antioxidant activity and high total phenol and flavonoid levels. Phyllanthus emblica fruit possesses antioxidant, immunomodulatory, and anticancer properties, Analgesic, anti-pyretic, antidiabetic, and antimicrobial.[1,2,3,4,5,6]

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