Abstract
In this study, we describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 25 pesticides in grape juice. This method was optimized and validated according with INMETRO (Instituto Nacional de Metrologia Qualidade e Tecnologia) for the simultaneous determination of pesticide, which are widely used in grape culture in Brazil. The method was based on the QuEChERS (quick, easy, cheap, effective, rugged and safe) methodology. We used AOAC Official Method 2007.01 and Standard Method EN 15662 for sample preparation, both methods presented statistically equivalent recoveries and coefficients of variation. At 0.005 and 2 mg L-1, fortified blank grape juices had average recoveries of 101 and 112% and average coefficients of variation of 16 and 11%, respectively. The repeatability was tested in three concentrations 0.005, 0.1 and 2 mg L-1, affording coefficients of variation 16.4, 20.7 and 10.8%, respectively.
Highlights
During production, processing, storage, and transport of food a variety of residues and contaminants may enter the food chain
There are several multi-residue methods, there were few methods developed for pesticides widely used in specific culture and region
Rebelo et al.[14,15] developed a LC-MS/MS multi-pesticide method, for pesticides which were widely used for rice culture in Brazil
Summary
During production, processing, storage, and transport of food a variety of residues and contaminants may enter the food chain. Crops are treated with pesticides and against pests and may leave residues in products of plant.[1] determination of pesticide residues in food matrices has become a necessity in view of the toxicity and stability of these xenobiotics.[2]. Due to the low detection levels required by the regulatory bodies and the complex nature of the matrices, which the target compounds are present, the trace level detection and identification with prior efficient sample preparation are important aspects in the analytical method.[3]. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the most powerful techniques for the analysis of pesticides in a variety of complex matrices.[3]. Liquid chromatography-mass spectrometry (LC‐MS) is preferred over gas chromatography (GC)
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