Abstract
A sensitive technique is described for the rapid, direct determination of normal levels of lithium and strontium in 100-μl samples of human blood serum without separation or preconcentration. Nitrous oxide—acetylene flame emission spectrometry, using conventional atomic absorption apparatus is used, with discrete sample injection. Lithium and strontium standards were prepared in 21% (v/v) glycerol which approximates the viscosity of serum. It is recommended that serum samples be analyzed by either calibration with artificial serum and glycerol standards or by immediate standard microaddition procedures. Results for pooled human serum indicate accuracy and precision of better than 6% at the 10 ng ml -1 level. The method is free from nebulizer clogging and matrix interferences and should be useful as a routine clinical laboratory procedure.
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