Abstract
4′-O-methylpyridoxine (MPN) and its 5′-glucoside (MPNG) in Ginkgo biloba seeds cause poisoning. Sample preparation for high-performance liquid chromatography was optimized to determine the amounts of MPN and MPNG at various extraction temperatures and pH levels. The effects of heating treatment on MPN and MPNG were investigated. MPNG was the main MPN analog in raw G. biloba seeds under the optimal temperature (0 °C) and pH (2.5). Boiling decreased the contents of MPN (81.76%), MPNG (9.15%), and total MPN (TMPN, 30.14%). The contents of MPN and TMPN considerably decreased (80.41% and 20.11%, respectively), whereas that of MPNG increased (4.42%) after microwave treatment. Results demonstrate that boiling can reduce the toxicity of G. biloba seeds for consumption.
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