Abstract
GLC and fluorometric procedures were developed for the determination of mycophenolic acid and its principal metabolite, mycophenolic acid glucuronide, in plasma. The GLC procedure consists of extraction of the compound, silylation with trimethylsilylimidazole in carbon disulfide, and chromatography of the sample on a 2% methylphenylsilicone fluid column at 270°. The silyl derivative of lithocholic acid is used as an internal standard. The fluorescence procedure consists of an extraction followed by measurement of the fluorescence of the compound in pH 10.0 buffer, with an excitation wavelength of 350nm. and an emission wavelength of 438nm. The accuracy of both procedures is better than 10%.
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