Determination of multi-drug resistant Klebsiella pneumoniae isolated from UTI patients in Wasit province, Iraq

Abstract

The noticeable increase in the occurrence of multidrug-resistant Klebsiella pneumoniae strains separated from different hospitals in Wasit Province-Iraq demonstrates the limitation of antibiotics used for bacterial eradication. The aim of the present study is to detect the virulence genes in some K. pneumoniae isolates that collected from different hospitals in Wasit Province-Iraq. A total of 525 clinical samples were used to isolate 77 K. pneumoniae strains from clinical specimens throw fife months. They were identified by microbiological method as K. pneumoniae. The antimicrobial susceptibility of K. pneumoniae isolates was determined. The existence of virulence genes (AcrAB and tolC) were performed by PCR. The multidrug-resistant isolates showed resistance against Gentamicin (3.89%), Tobramycin (15.58%), and Amikacin (12.98%), which belongs to aminoglycoside antibiotics. Morever, βeta-Lactam antibiotics include Penicillins: Ampicillin (100%), Augmentin (19.48%), Carbapenems class including: Polymyxin-B (2.59%), and Imipenem (19.48%), Tetracyclins represented by Tetracycline (27.27%), Quinolones class including Ciprofloxacin (19.48%), Levofloxacin (14.28%), and Norfloxacin (14.28%), Macrolidies class including Azithromycin (57.14%),Amphenicols class including Chloramphenicol (1.29%),Nitrofurans class including Nitrofurantion (57.14%), finally Co-Trimoxazole class including Trimethoprim (33.76%). Molecular diversity between K. pneumoniae isolates was determined using Multiplex PCR technique.