Abstract
To develop an efficient microspore culture system for broccoli, the effect of the developmental stage of the cultured microspore population was clarified, and embryogenic microspores were selected by percoll density gradients. The mean number of microspore nuclei was more effective than bud size for the determination of the developmental stage and microspore population that would be embryogenic. A microspore population having an average of 1.54-1.84 nuclei produced a high yield of embryos. Microspores selected by percoll (28/36%) produced about 3-fold the number of embryos of those that were not selected.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
