Determination of Insecticide Residues in the Air at the Breathing Zone While Exposure the Long Lasting Insecticidal Net Using Gas Chromatography with Mass Spectrometry - (GC-MS/MS)

Abstract

Long-lasting insecticidal nets (LLIN) are highly effective tools for preventing malaria infection and reducing associated morbidity and mortality [1]. Vector management chiefly through the usage of insecticide inside recurring spraying (IRS) as well as durable insecticidal nets (LLINs). The concept of utilizing insecticide-treated bed nets came during World War II, when Germans, Russian, and US armies began dealing with their combat uniforms of theirs as well as bed nets to safeguard them against vector-borne problems, primarily malaria as well as leishmaniasis [2]. There are many kinds of nets accessible. They differ by size, material, or therapy. The majority of nets are produced from polyester but nets can also be obtainable in cotton, polyethylene, or maybe polypropylene [3,4]. These insecticides are proven to pose really low health consequences to other mammals and humans, but are extremely poisonous to insects and knock them down, while at really low doses [5]. Mosquitoes drawn towards the LLIN due to the attraction of host (Personnel sleeping down the net), on landing the Insecticide present on the nets knockdown and kill the mosquitoes [6]. The health consequences from the LLINs themselves haven't been properly investigated as well as reported in the peer-reviewed scientific literature. A scientifically rigorous, objective, and also a transparent examination of the risks related to LLINs is necessary to give risk-benefit considerations from this promising technological innovation. Pyrethroids present in the air even they are semi-volatile organic compounds. The pesticide inhalation exposures from the bed net were estimated based on indoor Bomann study [7] The level of breathing zone pesticide was estimated this experiment and determined the concentration of active ingredient present in the breathing zone while using the Deltamethrin 1.52 g/kg coated onto filaments Polyester Long Lasting insecticidal Net. The possible daily systemic dose likely to prevail at the breathing zone due to the inhalation of insecticide while sleeping under the treated net is determined. The experiment was conducted in a chamber of size 10 ft x 10 ft x 10 ft (L x B x H). The net sample was fixed in the room following the routine usage pattern. The indoor air was sampled at an interval of 0 hr (5 minutes after experiment initiation), 2 hr, 4 hr, 9 hr, 10 hr, 11 hr, 12 hr and 14 hr. The air sampling was done at a constant flow rate of 5 L/min for 5 minutes (25 L of air). At each sampling occasion the air was suctioned through silica cartridges from four pre-determined locations of the room using suction pump assembly having air rotameters. The experiment was repeated for three days. The residue of Deltamethrin in air was determined for a period of 14 hours in static conditions (no air exchange in the experimental chamber) and dynamic condition at an air exchange rate 0.5 AER/h.