Abstract
Hydrogen peroxide (H 2O 2) concentrations in antiseptic solutions (normally 3% H 2O 2) has been determined non-destructively using a portable near-infrared (NIR) analyzer. The spectral variation due to OH band around 1400 nm in the second derivative spectra has been found as H 2O 2 concentration changes. Both multiple linear regression (MLR) and partial least squares (PLS) were employed to generate calibration models over the 1100–1720 nm range. The PLS calibration model showed the better calibration performance with a standard error of prediction (SEP) of 0.16%. In order to validate the developed PLS calibration model, H 2O 2 concentrations in commercial antiseptic solutions were predicted and compared with values from a conventional redox titration method. The results showed that NIR predictions had good correlation with conventional analysis values. The rapid and non-destructive determination of H 2O 2 in the antiseptic solution was successfully performed using portable NIR analyzer without any hazardous chemical solvents.
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