Abstract
AbstractA substance that causes hemolysis (breakdown of red blood cells) is known as hemolysin. The presence of safety-related virulence factors like hemolysin production is determined by hemolytic activity. The hemolysin produces a clear β-hemolytic zone on a blood agar plate. Blood agar contains general nutrients, and 5% defibrinated mammalian blood (human, sheep, or horse). Therefore, it is useful for the cultivation of pathogenic microorganisms and determining the hemolytic capabilities of an organism. Hemolytic reactions are evaluated by observing both the partial hydrolysis of the red blood cells and the production of a green zone (α-hemolysis), as well as the total hydrolysis of red blood cells producing a clear zone around the bacterial colonies (β-hemolysis) or no reaction (γ-hemolysis).Key wordsBlood agarDefibrinated bloodHemolysinHemolysisProbioticsα-hemolysisβ-hemolysisγ-hemolysis
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