Abstract
A new method for the determination of hemoglobin (Hb) is described. It is based on the amplification of the Hb enzyme-catalyzed reaction between O-phenyldiamine (OPD) and H2O2 and polarographic measurement of the enzyme-generated product 2,2′-diaminoazobenzene (DAA). A linear calibration plot has been obtained within the range of 1∼20 μg/ml (r = 0.987) Hb. The assay is performed on the microtitre plate to avoid the influence of serum protein and to obtain good reproducibility. The method is practical, reliable and convenient, and has been applied to determine Hb in plasma and serum.
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