Abstract
A coupled-column liquid chromatographic method for determining the free concentration of ropivacaine and bupivacaine in blood plasma was developed. Following adjustment of the temperature and pH, the plasma samples were ultrafiltrated. Ropivacaine or bupivacaine in the ultrafiltrate was determined by direct injection into a coupled-column liquid chromatographic system, consisting of one reversed-phase and one ion-exchange column. The system was highly selective. Ropivacaine and bupivacaine were detected by UV at 210 nm. The limit of determination was 10 nM and the inter-assay precision at a concentration level of about 100 nM was 6% (R.S.D., n = 30) for ropivacaine and 7% (R.S.D., n = 30) for bupivacaine.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of chromatography. B, Biomedical applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
