Abstract
In order to investigate the possible use of the dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) ratio as a stress indicator of Posidonia oceanica a method for the determination of these quantities was developed for this type of material.•The method relies on gas chromatography with headspace technique, instead of the purge-and-trap technique commonly used.•The method allows the determination of both DMSP and DMSO on the same sample.•This method allows to quantify DMSP, DMSO and DMSP:DMSO ratio for calibration curves with a coefficient of variation around 2% and a relative error around 2% and within the ranges natural variability of DMSP and DMSO in P. oceanica leaf tissue.Preliminary tests showed that DMSP in P. oceanica leaf tissue ranged from 20 to 200 μmol g−1 of fresh weight (FW) and 2 to 5 μmol gfw−1 for DMSO. The DMSP:DMSO ratio ranged from 2 to 40. The quantifications were conducted with different mixtures of DMSP and DMSO by measurements of DMSP and DMSO in the same sample of P. oceanica leaf tissue.
Highlights
The method relies on gas chromatography with headspace technique, instead of the purge-and-trap technique commonly used
DMSP is the precursor of dimethyl sulfide (DMS) and acrylic acid; these two compounds can act as herbivore deterrent for macrophytes [3]
The method we propose here is an adaptation of DMS(P,O) analytical method that is used typically for phytoplankton [7], that was adapted for P. oceanica leaf tissue, and is based on the headspace technique, instead of purge-and-trap
Summary
The method relies on gas chromatography with headspace technique, instead of the purge-and-trap technique commonly used. We have shown that P. oceanica leaf tissues can have a high concentration of DMSP (up to 200 mmol gÀ1 of fresh weight (fw)) and DMSO (up to 2 mmol gfwÀ1) [2].
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