Abstract
The determination of heavy metal concentrations has been carried out using sophisticated instruments, and therefore a simple and reliable alternative method is needed as a comparison. The study aimed to determine Cu and Pb concentration of standard solution using the urease activity inhibition method of Durio zibethinus L. seeds. The research started with urease extraction from Durio D. zibethinus L. seeds. The activity of the obtained extract was determined using the Nessler method. The optimum substrate concentration was also determined. Urease activity inhibition was carried out using various metal solution concentrations, which continued by plotting a log graph of urea concentration vs. %inhibition. The obtained graph would then determine the metal concentration in a synthetic water sample. The data was then compared to the measurement, determined by the Atomic Absorption Spectrophotometry (AAS) method. Results of the study showed that the urease activity of D. zibethinus L.seeds was 296.774 U/mL. Urease activity was optimum at a urea concentration of 0.3 M. The comparison Cu, and Pb concentration determination using the urease inhibitory activity and AAS methods showed no significant difference at 95% confidence level. This research showed that urease of D. zibethinus L. seed could be used to determine Cu and Pb's concentration based on its inhibiting activity.
Highlights
Heavy metals can cause environmental pollution, and this is a big problem because they affect human health
Various methods have been developed to determine the concentration of heavy metals, such as atomic absorption spectrometry (Souza, Zanatta, da Silva, & da Veiga, 2018), inductively coupled plasma mass spectrometry (Djedjibegovic, Larssen, Skrbo, Marjanović, & Sober, 2012), anodic stripping voltammetry (Bernalte, Sánchez, & Gil, 2011) and X-ray fluorescence spectrometry (Aranda, Colombo, Perino, De Vito, & Raba, 2013); (Ozer, Çimenoğlu, & Güçer, 2011)
The study results show that the percentage of inhibition of the urease activity of D. zibethinus L. seed increases with increasing metal ion concentration
Summary
Heavy metals can cause environmental pollution, and this is a big problem because they affect human health. Keywords : Durio zibethinus L. seed, urease activity inhibition, Cu and Pb concentration At constant substrate concentrations and varying metal ion concentrations, inhibition causes the enzyme catalytic activity to decrease.
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