Abstract
A method was developed for the determination of chromium as the Cr(acac) 3 complex in urine using SFE and chromatography. Quantitative extractions were achieved when the experiments were carried out under 3000 p.s.i. of pressure, at a temperature of 120 °C, with 2.0 ml of methanol, 30 min of static extraction and 5 min of dynamic extraction. The chromium was quantified by GC–FID detection. The calibration graph of Cr(acac) 3 solutions was linear between 0.50 and 43.0 μg ml −1 of chromium (DL 0.18 μg ml −1, R=0.9994). The same extracts were quantified by HPLC-340 nm detection. The calibration curve of the Cr(acac) 3 solutions was linear over a range of 0.013 to 60.0 μg ml −1 (DL 0.02 μg ml −1, R=0.9999). This method was applied to urine samples from 60 diabetic patients and 21 healthy volunteers. Chromium concentration ranges were 2.5–29.5 μg l −1 for the diabetics and 5.9–12.3 μg l −1 for the normal subjects.
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