Abstract
In this research, we aimed to detect bla VIM and bla IMP the resistant genes against Meropenem in Pseudomonas aeruginosa from sample of hospitalization patients at the Internal Medicine HCU of RSUP DR. M. Djamil Padang. Firstly, bacterial isolate of P. aeruginosa were isolated from the sputum samples of patients who suffered bronkopneumonia. The isolation were started with samples cultivation to the Cetrimide Agar media which was a selective media for P. aeruginosa . To determine the species of the bacteria, the identification using Gram staining, Triple Sugar Iron Agar (TSIA) test, citric test, urease test, Methyl Red/ Voges–Proskauer (MR/VP) test, and molecular marker of 16S rRNA genes have been conducted. The isolation and identification result showed that from 20 sputum samples of the patients there were just 10 (50%) samples were positively containing P. aeruginosa . From the P. aeruginosa isolates, the resistant genes against meropenem bla VIM and bla IMP were amplified using PCR. The result showed that all these P. aeruginosa isolates have positively genes encoding for Metallo-β-Lactamase (MBLs).
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