Determination of basophil activation and IgE antibodies to the components of dental materials in cases of intolerance to dental prosthetics

Abstract

The aim of the study was to determine the activation of blood basophils and IgE antibodies to the components of dental materials (CDM) in patients with intolerance to dental prosthetics. Materials and methods: we examined 22 patients with positive skin application tests (AT) with metal salts solutions (NiCl2 (3%), CrCl3 (3%), CoCl2 (1%) and complaints of intolerance to dental materials (IDM), and 21 patients without IDM complaints. We determined the levels of CD203c+CD63+ basophils after incubation of blood with incubation liquid containing particles of prosthetics (LPP) and metal salts solutions (MSS): NiCl2, CrCl3, CoCl2, ZnCl2, MnCl2, also measured IgE antibodies to these salts using ELISA. Results: in patients with IDM and skin hypersensitivity to metal salts an increase or decrease of CD203c+basophils after incubation of basophils with LPP and MSS occurred. In patients with skin hypersensitivity to CDM basophils significantly more often and stronger reacted with LPP and metal salts compared to basophils of control group patients. It was established that LPP had the highest diagnostic efficiency (sensitivity 72,7%; specificity 90,5%; AUC 0,86; p=0,0003; with threshold of expression growth for CD203c+63+ basophils equaling 16,68%), compared to MSS as basophil activation inductor. Conclusion: basophil activation test and IgE antibodies determination are recommended for detection of hypersensitivity and allergy in patients with IDM.