Abstract
This study describes the preparation and application of a poly (3, 4-Ethylenedioxythiophene) (PEDOT) modified glassy carbon electrode for ascorbic acid determination in fruit samples by square wave voltammetric method. The PEDOT modified electrode was prepared by electrochemical polymerizing of 3, 4-Ethylenedioxythiophene (EDOT) on a glassy carbon electrode in a non-aqueous medium (0.1 M Tetrabutylammoniumhexafluorophosphate and 0.1 M EDOT in acetonitrile). The best performance of the PEDOT modified electrode in 0.1 M phosphate buffer was obtained at pH 5.0. In addition to this other parameters like electrode thickness, square wave amplitude, and square wave frequency were optimized. Under these conditions, an oxidation potential of ascorbic acid was observed at 395.24 mV versus Ag/AgCl/ reference electrode. The square wave voltammetric study indicated that the PEDOT modified electrode showed a very good electrocatalytic activity by reducing the overpotential by 113.69 mV for ascorbic acid oxidation. The modified electrode also showed a linear response range between 50 and 90μM. The proposed method was checked by determining the recovery of ascorbic acid in two commercially available tablets and provided an excellent recovery (i.e. 99.43 and 93.80%). Finally the method was applied in Orange and Pineapple fruits and 68.11±0.012 and 45.46±0.023 mg ascorbic acid per 100 g of the sample was found in each fruits respectively.
Highlights
Vegetables and fruits are part of man’s most important food categories being necessary for the maintenance of life and health
Cyclic voltammogram of 1.0 mM of ascorbic acid in phosphate buffer solution at bare glassy carbon electrode in which the electrochemical oxidation of ascorbic acid was observed with anodic peak current at 0.073 mA and high anodic potential at 508.93 mV and this was a large potential as compared to the potential obtained from modified electrode
Whereas curve (c) showed the catalytic oxidation of ascorbic acid at the glassy carbon electrode modified with PEDOT
Summary
Vegetables and fruits are part of man’s most important food categories being necessary for the maintenance of life and health. Vitamins are organic nutrients that are required in small quantities for a variety of biochemical functions They generally cannot be synthesized by the human body and must be supplied in diet. Vitamin C (ascorbic acid) is a water soluble antioxidant which helps protect against cancer, heart diseases and stress and which is found in variable quantities in fruits and vegetables [4]. A wide variety of Amanial Haile Reda and Fisseha Guesh Gebremeskel: Determination of Ascorbic Acid in Citrus Sinensis and Ananas Comosus Using Poly (3, 4-Ethylenedioxythiophene) Modified Glassy Carbon Electrode other foods contain sufficient quantities of vitamin C, such as Pineapples and Sweet peppers [7]
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