Abstract
Background and aims: Klebsiella is an opportunistic organism that is the cause of severe diseases such as pneumonia, septicemia, and urinary tract infections (UTIs). In addition, high antibiotic resistance has challenged the treatment of this bacterium. However, carbapenem antibiotics are considered as the therapeutic agents for selecting the treatment of penicillin- and cephalosporin-resistant gram-negative bacterial infections. The present study aimed to determine the resistance and minimum inhibitory concentration (MIC) of meropenem and imipenem. Methods: A total of 80 Klebsiella spp isolated from UTIs were collected in various educational wards (i.e., urology, obstetrics, and gynecology, as well as the units of infectious diseases, internal medicine, and intensive care) in different hospitals of Shahrekord. The isolates were then identified by using biochemical tests. Further, disc diffusion method was employed to determine the antibiotic resistance. Furthermore, MIC was estimated by the Epsilon-test strip. Moreover, P=Q=0.50, an error of 0.05, and an accuracy of 0.11 were considered for determining the sample size (n=80). Results: Based on the results of disc diffusion method, 24 strains were resistant to meropenem and imipenem. Additionally, the MIC was 24 (30%) by the E-test. In addition, 24 isolates had a MIC of ≥4 μg/mL for meropenem and imipenem and thus were resistant while 18 isolates were found to have a MIC of 1≤ MIC<4 μg/mL and therefore, were considered semi-sensitive (P<0.001). Conclusion: In general, Klebsiella strains were found to be resistant to meropenem and imipenem. Therefore, rapid and accurate identification of these strains and the selection of appropriate antibiotics can help quickly eradicate the infections caused by these bacteria. Accordingly, a waste of time, the consumption of medication, or even an increased resistance are prevented.
Highlights
Urinary tract infection (UTI) is the second leading reported infection in patients since nearly seven million patients are annually treated by the doctors [1]
In another study by Kanchanadevi et al in India, 25 out of 76 E. coli isolates, 2 out of 9 Klebsiella isolates, and 42 out of 60 Pseudomonas isolates were found to be resistant to imipenem based on the minimum inhibitory concentration (MIC) results by using E-test, which is approximately consistent with the results of the current study with respect to the number of imipenem-resistant Klebsiella isolates per the total number of the isolates, indicating a lower prevalence of carbapenem resistance in Klebsiella and E. coli compared to Pseudomonas [28]
All of these bacteria were examined for resistance to carbapenems, the results of which demonstrated that 20 (43.5%) Pseudomonas aeruginosa, 11 (23.9%) Acinetobacter baumannii, 3 (6.5%) E. coli, 2 (4.3%) K. pneumoniae, and 1 (2.2%) Enterobacter were resistant to carbapenems, which contradicts the results of the present study with respect to resistant Klebsiella isolates from the patients in the intensive care unit (ICU)
Summary
Urinary tract infection (UTI) is the second leading reported infection in patients since nearly seven million patients are annually treated by the doctors [1]. Klebsiella strains are one of the major bacterial causes of the UTI [2]. Determination of antibiotic resistance and mice of meropenem and imipenem growth in Klebsiella strains. Klebsiella is an opportunistic organism that is the cause of severe diseases such as pneumonia, septicemia, and urinary tract infections (UTIs). Carbapenem antibiotics are considered as the therapeutic agents for selecting the treatment of penicillin- and cephalosporin-resistant gram-negative bacterial infections. Methods: A total of 80 Klebsiella spp isolated from UTIs were collected in various educational wards (i.e., urology, obstetrics, and gynecology, as well as the units of infectious diseases, internal medicine, and intensive care) in different hospitals of Shahrekord. Results: Based on the results of disc diffusion method, 24 strains were resistant to meropenem and imipenem.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Shahrekord University of Medical Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
