Determination of an effective agent combination using nisin against Salmonella biofilm.

Abstract

This present study aims to determine the lowest concentration effects of the assayed different antibiotics; antimicrobial agents alone and their combinations with nisin were investigated to prevent the biofilm formation and break down the biofilm structure of Salmonella. While the combination of nisin and EDTA showed a synergistic effect against Salmonella strain, chlorhexidine digluconate and streptomycin with nisin showed a partial synergetic effect; citric acid and sulfonamides with nisin showed an indifferent effect. The use of citric acid and chlorhexidine digluconate alone was very effective in Salmonella inhibition. While the citric acid combined with other agents had not much effect, the use of chlorhexidine digluconate combined with nisin and EDTA inactivated the total initial count within 24h. Significantly, when citric acid and sulfonamides are used alone, they reduce by 64% and 44%, respectively. When they used nisin + EDTA, this ratio increased to 83% and 84%, respectively. For the prevention of biofilm, the most suitable conditions were determined as 97% biofilm inhibition. The results of this study can be used as a guide for the emergence of new approaches to ensure the food safety and quality of the food industry.