Abstract
A sensitive and simple method for the simultaneous evaluation of histamine, tryptamine, tyramine, phenylethylamine, putrescine, cadaverine, 1,6-diaminohexane and tryptophan in wine was developed using HPLC and coulometric detection without clean-up pretreatment. After precolumn derivatization with o-phthaldialdehyde the compounds were separated on a reversed-phase column with gradient elution. To increase the selectivity of the method a coulometric array of sixteen electrodes at increasing potentials was used. The total analysis time was less than 35 min. The analytical performance of the methods is reported. The method was used to examine the levels of various amines in three different wines.
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