Determination celiprolol hydrochloride drug by used zero, first, second and third order derivative and peak area spectrophotometry method in its pure form and in pharmaceutical tablets

Abstract

An easy, specified, accurate, precise and reproducible quantitative analyses for determination of celiprolol hydrochloride drug by used used zero, first, second and third order derivative and peak area spectrophotometry method. The suggest methods determined the drug in the concentration range (0.5-30) μg.mL−1, at 286.6 nm for 0th order, at 306.6 and 272.2 nm for 1st order, at 319.2, 289.8 and 250.2 nm for 2nd order and at 325.6, 304.8, 242.2 and 219.6 nm for 3rd order derivative spectrophotometry, respectively. The peak area spectrophotometry method also used in the same range for determining celiprolol hydrochloride, at (284.4-379.2) and (248.6-284.4) nm for 1st order, at (306.4-372.2), (271.2-306.4) and (239.4-271.2) nm for 2nd order, and at (318.6-363.8), (290.4-318.6), (233.2-250.4) and (210.8-233.2) nm for 3rd order, respectively. The accuracy and precision of the methods used was calculated and the results were highly satisfactory. The limit of detection (LOD) and limit of quantification (LOQ) was calculated for the suggested methods, Where (LOD) was within range (0.0124-0.0632) μg.mL−1, and (LOQ) within range (0.0415-0.1632) μg.mL−1. The methods were successful in application when estimating celiprolol hydrochloride drug on some pharmaceutical tablets available in the local markets.