Abstract
Ultraviolet radiation (UVR) is known to be mutagenic, cytotoxic, and carcinogenic (1,2,3). Cyclobutyl pyrimidine dimers induced in DNA by UVR have been implicated as a major cause of these deleterious biological effects (1,4,5,6). A more complete understanding of the etiological role of pyrimidine dimers in photo-carcinogenesis will be facilitated by direct measurement of these lesions in mouse skin (a model) as well as human skin. Several immunological methods for the detection of pyrimidine dimers have been developed that provide alternatives to chromatographic and enzymatic methods previously used to measure these lesions. The initial demonstration of experimentally induced antibodies directed against UV-irradiated DNA (UV-DNA) by Levine et al. (7), prompted a number of laboratories to produce anti-UV-DNA sera by traditional immunization procedures in rabbits (8–16) or by hybridoma methods (17). The present report describes the production, characterization, and use of monoclonal antibodies that are specific for thymidine dimers.
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