Abstract
BackgroundHydatidiform moles carry a significant risk for developing persistent gestational trophoblastic disease. Lectins are useful tools to identify cellular glycosylation pattern and changes in glycosylation that occur during growth, development, differentiation and also, during disease states.ObjectivesConsidering the changes in glycosylation that occur during cell proliferation, differentiation and transformation, the aim of the present study was to evaluate the sugar chain expression in hydatidiform mole by using HRP-conjugated lectins.Materials and MethodsLectin histochemistry with a panel of HRP-conjugated lectins comprising SBA, PNA, VVA, UEA-1, LTA, GS-І (B4) and WGA were performed in 20 molar (partial & complete moles) formalin-fixed, paraffin-embedded tissue samples.ResultsThe partial and complete moles generally showed similar reactivity with all used lectins. None of lectins reacted with villous cytotrophoblasts, whereas 4 of 7 lectins comprising WGA, LTA, UEA-І and PNA (after pretreatment with neuraminidase) showed a moderate to strong reactivity with villous syncytiotrophoblasts in both partial and complete hydatidiform moles. The villous stroma reacted with all used lectins except VVA.ConclusionsOur histochemical findings showed a relatively heavy glycosylation of syncytiotrophoblasts of both partial and complete molar tissues, which was prominent in apical portion. This may play a role in their capacity to increase trophoblastic proliferation.
Highlights
Hydatidiform moles carry a significant risk for developing persistent gestational trophoblastic disease
The villous stroma showed moderate reactivity (Figure 1 c), after neuraminidase treatment moderate reactivity was observed in syncytiotrophoblasts, which was most pronounced in apical portion .The reactivity of other components of villi did not altered (Figure 1 d)
Our results indicate that HRP-conjugated lectins used in this study react differentially with various components of molar tissue; these reactions were generally similar in partial and complete hydatidiform moles
Summary
Hydatidiform moles carry a significant risk for developing persistent gestational trophoblastic disease. Lectins are useful tools to identify cellular glycosylation pattern and changes in glycosylation that occur during growth, development, differentiation and during disease states. Conclusions: Our histochemical findings showed a relatively heavy glycosylation of syncytiotrophoblasts of both partial and complete molar tissues, which was prominent in apical portion This may play a role in their capacity to increase trophoblastic proliferation. Implication for health policy/practice/research/medical education: Lectins are useful tools to identify cellular glycosylation pattern and changes in glycosylation that occur during growth, development, differentiation and during disease states. This study focuses on the lectin binding pattern in hydatidiform mole, which may play a role in the increased trophoblastic proliferation that occurs in this most common form of gestational trophoblastic disease.
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