Abstract
RFLP analysis was used on several meat sources to identify the species. It might be important to evaluate a method to identify different meat species. We tested Buffalo, Cattle, Goat and Sheep to enforce labeling regulation in the authentication of each of them. In this study, the presence of restriction sites on the polymerase chain reaction (PCR) products has been used for the specific identification of the four different meat species. The cytochrome b encoding gene (359 bp) as a part of the mt-DNA was amplified. The digestion of the PCR products using Anil and Hae III gave specific restriction profiles that allowed a direct identification of farm animal species in raw and heat-treated meat samples. The results showed that RFLP analysis provided with a rapid and effective method to detect meat species and could be easily used for meat identification and authentication.
Highlights
DVFHUWDLQ WKH VSHFLHV XVHG LQ WKH PDQXIDFWXUH RI WKRVH SURGXFWVPRGHUQ WHFKQLTXHV QRZ DOORZ WKH LGHQWLILFDWLRQ RI VSHFLHV VSHFLILF 'HWHFWLRQ 2I 6SHFLHV 6SHFLILF *HQHWLF 0DUNHUV ,Q )DUP $QLPDOV.
WZR PDMRU DGYDQWDJHV RYHU SURWHLQ DQDO\VLV VDPSOHV KHDWHG WR DV KLJK DV R & FDQ VWLOO EH DQDO\]HG DQG GLVFULPLQDWLRQ EHWZHHQ UHODWHG VSHFLHV.
DPRQJ SRSXODWLRQV VWXGLHG $ FRPELQDWLRQ RI 3&5 DPSOLILFDWLRQ RI PW '1$ DQG UHVWULFWLRQ IUDJPHQW OHQJWK SRO\PRUSKLVP 5)/3 DQDO\VLV KDV.
Summary
PRGHUQ WHFKQLTXHV QRZ DOORZ WKH LGHQWLILFDWLRQ RI VSHFLHV VSHFLILF 'HWHFWLRQ 2I 6SHFLHV 6SHFLILF *HQHWLF 0DUNHUV ,Q )DUP $QLPDOV. WZR PDMRU DGYDQWDJHV RYHU SURWHLQ DQDO\VLV VDPSOHV KHDWHG WR DV KLJK DV R & FDQ VWLOO EH DQDO\]HG DQG GLVFULPLQDWLRQ EHWZHHQ UHODWHG VSHFLHV. DPRQJ SRSXODWLRQV VWXGLHG $ FRPELQDWLRQ RI 3&5 DPSOLILFDWLRQ RI PW '1$ DQG UHVWULFWLRQ IUDJPHQW OHQJWK SRO\PRUSKLVP 5)/3 DQDO\VLV KDV.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
