Abstract
The aim of the present work was to identify the possibility of immunocytochemical detection of various tissue antigens on paraffin sections of rat spinal cord surrounded by spinal bony tissue and adjacent muscles fixed in zinc-ethanol-formaldehyde after decalcification with formic acid in adult (n = 5) and neonatal (n = 2) Wistar rats. These studies used antibodies to glial fibrillary acidic protein, neurofilaments, neuronal nuclear protein, tyrosine hydroxylase, the synaptic vesicle protein synaptophysin, and the intermediate filament protein vimentin. The combination of fixation in zinc-ethanol-formaldehyde and decalcification in 21% formic acid solution provided optimal preservation of the antigens of interest.
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