Abstract
The lipase in fresh rice bran was used to hydrolyze the ester bond of Tween on gold nanoparticles colorimetric sensor to detect the lipase activity in rice bran. The characterization showed that the Zeta value of gold nanoparticles changed from −16.58 mV to −3.56 mV and the gold nanoparticles shifted to rapid aggregation. When the lipase activity was in the range of 0.33 U/ml to 56.8 U/ml, the detection limit was 0.0528 U/ml. The lipase activity in fresh rice bran detected by gold nanoparticles colorimetric sensor was highly correlated with potentiostatic titration (R2 = 0.999). The relative deviation of A650/A520 obtained by adding lipoxygenase, peroxidase and esterase was less than 5%. Six gold nanoparticles colorimetric sensors developed in the same batch were used to detect the lipase activity in rice bran samples. The gold nanoparticles colorimetric sensor modified by Tween 20 was accurate and reusable.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
