Abstract
A protocol was set up to detect latent infections of Ralstonia solanacearum biovar 2, race 3 in field tomatoes. For two growing seasons, healthy tomato plants were inoculated with a virulent strain, and monitored for between 7 and 55 days. Final extracts used for direct isolation on Kelman’s medium and for indirect immunofluorescence staining were prepared from 1 cm segments collected from the base of the lowest side shoots. Reisolations were identified by colony morphology, PCR, IFAS, and pathogenicity tests on tomato plantlets. Reisolation was successful from 18 days onwards, with a frequency that constantly increased in the following weeks. Samples prepared by mixing one latently infected segment with increasing numbers of healthy segments revealed a sensitivity threshold of 1: 999. This non-destructive protocol was shown to be appropriate for monitoring in the open field.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
