Abstract
The present study was carried out to assess the detection sensitivity of four microbial inhibition assays (MIAs) in comparison with the results obtained by the High Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) method for antibiotics of the β-lactam group and chloramphenicol in fortified raw milk samples. MIAs presented fairly good results when detecting β-lactams, whereas none were able to detect chloramphenicol at or above the permissible limits. HPLC analysis revealed high recoveries of examined compounds, whereas all detection limits observed were lower than their respective maximum residue limits (MRL) values. The extraction and clean-up procedure of antibiotics was performed by a modified matrix solid phase dispersion procedure using a mixture of Plexa by Agilent and QuEChERS as a sorbent. The HPLC method developed was validated, determining the accuracy, precision, linearity, decision limit, and detection capability. Both methods were used to monitor raw milk samples of several cows and sheep, obtained from producers in different regions of Greece, for the presence of examined antibiotic residues. Results obtained showed that MIAs could be used effectively and routinely to detect antibiotic residues in several milk types. However, in some cases, spoilage of milk samples revealed that the kits’ sensitivity could be strongly affected, whereas this fact does not affect the effectiveness of HPLC-DAD analysis.
Highlights
Antibiotics are widely used in veterinary practice for the treatment of microbial infections or diseases and as dietary supplements
The antibiotic residues’ analysis is important to guarantee food safety [1]. Regulatory authorities such as the European Union (EU) and the US Food and Drug Administration, in order to ensure food safety, have enacted strict Maximum Residue Limits (MRLs) that allow only trace amounts of antimicrobial residues in milk for human consumption, and requirements concerning the performance of analytical methods and the interpretation of the results [1]
Absolute recoveries after ultrasound assisted matrix solid-phase dispersion (MSPD) ranged from 75–94% for all compounds
Summary
Antibiotics are widely used in veterinary practice for the treatment of microbial infections or diseases and as dietary supplements. The excessive and inconsiderate use of antibiotics may lead to the occurrence of drug residues in milk, bearing a risk to human health, since they can cause allergic reactions in hypersensitive individuals, or they may result in drug-resistant bacteria. The antibiotic residues’ analysis is important to guarantee food safety [1]. Regulatory authorities such as the European Union (EU) and the US Food and Drug Administration, in order to ensure food safety, have enacted strict Maximum Residue Limits (MRLs) that allow only trace amounts of antimicrobial residues in milk for human consumption, and requirements concerning the performance of analytical methods and the interpretation of the results [1]. Beta-lactam antibiotics are among the most frequently used antimicrobial agents, and include penicillin derivatives, cephalosporins, monobactams, carbapenems, and β-lactamase inhibitors.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
