Detection of IL1β Gene in Sudanese Patients with Gliomas Tumor in Khartoum state, Sudan

Abstract

Introduction: Gliomas are the most prevalent type of primary intracranial tumors, representing 80% of all brain neoplasms.In Sudan Brain tumors have been as leading cause of mortality among children and as thirdcausative death among young adults.World Health Organization (WHO) classification2021, categorized gliomas into lower-grades gliomas (LGG,grades II and III) andglioblastoma (GBM, grade IV).Gliomas are surrounded by pool of pro inflammatory cytokines, chemokine and growth factor, particularly review the tumorigenic effects of central nervous system one of this inflammatory cytokines is Interleukin-1ß (IL-1ß ) which play a crucial role in gliomas pathophysiology.
 Aims of the study: This study aimed to amplify IL-1ß gene and detect -5511, rs371339015 and rs376341819 SNPs in gliomassubtypes among Sudanese patients using PCR and Sanger sequencing.
 Material and methods: The study was conducted at the National Center for Neurological Sciences during the period from May to September 2022, Khartoum Sudan. From all gliomas patientsduring the above mentioned period, tissue samples were collected and processed for DNA extraction, PCR and further Sanger sequencing
 Results: In this study, the most common affected agegroup was 31- 40 years, supratentorial location was seen in more than half of the patients and the astrocytoma grade 1 was detected in 80.9%.
 In astrocytoma grades I, the most frequent mutation was C >A in 28.7% and A > C in 19%.
 C>T mutation was present in gliomasgrade I, II and III.
 Conclusions: In this study, C>T was the most encountered mutation in astrocytoma grades.
 Key words: Interleukin-1ß, DNA, PCR