Detection of Hepatocystis sp. Infection in Vervet Monkeys (Chlorocebus Pygrythrus) Imported from Tanzania Using Molecular and Microscopic Methods.

Abstract

The present study examined and reported Hepatocystis sp. infection in wild-caught vervet monkeys imported from Tanzania into the Razi vaccine and serum research institute (RVSRI). Polymerase chain reaction (PCR) targeting of 18S rRNA gene, followed by sequencing, Basic Local Alignment Search Tool (BLAST), and phylogenetic studies revealed that 82.8% of the imported monkeys were infected with Hepatocystis. Nevertheless, as illustrated by a routine parasitological examination of blood smears and histopathological examination of liver collected samples, the rates of Hepatocystis infection were obtained at 33.9% and 38.1%, respectively. Two isolated 18S rRNA gene sequences of Hepatocystis sp. from Tanzanian vervet monkeys were registered under the accession numbers OM281567 and OM281564 in GenBank. Although Hepatocystis infections do not cause clinical disease, they may interfere with the research data. The results of the current study pointed out that after proper nutrition and implementation of good physical environmental conditions for 3-4 months, the imported monkeys obviously gained weight and most of their hematological parameters, even in the presence of the parasite, returned to the normal levels and the experimental monkeys would be ready for use in studies.