Abstract
Objective:- Hepatitis B is noteworthy medical issues that may include the late continuation of liver cirrhosis and hepatocellular carcinoma. The present study aimed for the detection and diffrentiation of Hepatitis B virus HBsAg inactive non-replicative carriers, HBeAg-positive inactive replicative carriers, active carriers & HBeAg-negative chronic hepatitis B by Real Time PCR and their genotyping
 Methods: This research conducted on 245 positive for HBsAg, 118 (48.16 %) were male and 127 (51.84%) were female patients, which was performed in central research station labortory of Microbiology at netaji subhash Chandra Bose subharti Medical College and Hospital, Meerut Between march 2016 to November 2017 The sera were separated and screened for HBsAg by ELISA kit. Positive samples for HBsAg were tested for HBeAg ELISA kit and DNA Viral load then sequenced for genotying
 Results:. Of the 245 HBsAg Positive case 55 (1.12%) were HBeAg positive. In 16 PCR positive and HBV genotyping, In HBsAg inactive Non-Replicative 37.5% (n=6) genotype-B and 6.25% (n=1) genotype-A, In HBeAg inactive Replicative 12.5% (n=2) genotype-B and 12.5% (n=2) genotype-A and In HBeAg Active Chronic Hepatitis B 18.75% (n=3) genotype-B and 12.5% (n=2) genotype-A were detected
 Conclusions: Management strategy, using HBsAg, HBeAg and HBV DNA viral load, seems adequate for the confirmation and diffrentiation of Hepatitis B virus inactive, active carriers & HBeAg-negative chronic hepatitis B patients and genotype B was more prevalent in comparission to genotype A. Distribution of carriers & genotypes, help physicians to prescribe proper antiviral/interferon therapy according to current genotyping pattern in this region
 Keywords: Hepatitis B virus, Carrier State, HBsAg, HBeAg, RT-PCR
Highlights
The human hepatitis B virus (HBV) is a smallenveloped DNA virus causing acute and chronic hepatitis
The present study aimed for the detection and diffrentiation of Hepatitis B virus HBsAg inactive non-replicative carriers, HBeAg-positive inactive replicative carriers, active carriers & HBeAg-negative chronic hepatitis B by Real Time PCR and their genotyping Methods: This research conducted on 245 positive for HBsAg, 118 (48.16 %) were male and 127 (51.84%) were female patients, which was performed in central research station labortory of Microbiology at netaji subhash Chandra Bose subharti Medical College and Hospital, Meerut Between November 2016 to April 2018
All 245 HBsAg positive cases was tested for HBeAg and DNA amplification to detect HBsAg inactive non-repalicative carriers, HBeAgpositive inactive repalicative carriers, HBeAgpositive active chronic hepatitis B carriers & HBeAg-negative chronic hepatitis B carriers
Summary
The human hepatitis B virus (HBV) is a smallenveloped DNA virus causing acute and chronic hepatitis. Notwithstanding the availability of a secure and safe vaccine, HBVIstill represents a first-rate global fitness burden, with approximately 350-400 million human beings chronically infected global and about 6 lakh deaths in step with 12 months. Between 15 and 40% of chronically infected people may increase intense liver disease and hepatocellular carcinoma (HCC), whilst the closing turn out to be inactive carriers. Hepatitis B carriers are about 5% of the total world’s population, defined as being positive for HBsAg. HBV is endemic in lots of areas of the arena, which consist of Asia, Micronesia, and sub-Saharan Africa in addition to in a few populations in Australia, New Zealand, South the United States, the center east and the Arctic. An anticipated 1.25 million human beings in the US are HBsAg Positive (HBsAg-P). Fifteen % to 40 % of those carriers may expand hepatitis B-related sequelae of their lifetimes [3,4,5]
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