Detection of glutamate and glutamine (Glx) by turbo spectroscopic imaging

Abstract

Turbo spectroscopic imaging (TSI) is a spin echo spectroscopic imaging technique in which two or more echoes are acquired per excitation to reduce the acquisition time. The application of TSI has primarily been limited to the detection of uncoupled spins because the signal from coupled spins is modulated as a function of echo time. In this work we demonstrate how the TSI sequence can be modified to observe spins like the C 2 protons of Glx (≈3.75 ppm) which are involved solely in weak-coupling interactions. The technique exploits the chemical shift displacement effect by employing TSI refocusing pulses that have bandwidths which are less than the chemical shift difference between the target spins and the spins to which they are weakly coupled. The modified TSI sequence rewinds the J-evolution of the target protons in the slice of interest independently of the echo time or echo spacing, thereby removing any signal variation between successive echoes (apart from T 2 relaxation effects). In this study we tailored the narrow-bandwidth TSI sequence for observation of the C 2 Glx protons. The echo time was experimentally optimized to minimize signal contamination from myo-inositol, and the efficacy of the method was verified on phantom solutions of Glx and on brain in vivo.