Detection of EGFR mutation in tissue and peripheral blood by denaturing high performance liquid chromatography (DHPLC) for patients with advanced non-small cell lung cancer

Abstract

11013 Background: Goal of this study is to apply DHPLC as a screening system of detection of EGFR mutation (M) for large scaled population, and to evaluate the correspondence between EGFR M in tissue(T) and in matched serum DNA and their clinical significance in ANSCLC Methods: 230 primary NSCLC T and matched peripheral blood samples(MPBS) were obtained. Special polymerase chain reaction (PCR) of EGFR exon 19 and 21 were performed After DNA extraction. DHPLC was applied to detect the M status in matched samples which were confirmed by standard DNA sequencing. Results: EGFR M was found by DHPLC to be 34.3%(77/230) in T and 34.3%(79/230) in MPBS. 70 pair samples with and without EGFR M were confirmed by sequencing. The sensitivity and specificity of DHPLC of detecting EGFR M were 95% and 93%, respectively, the correlation index(CI) was 0.87 (P<0.001). Among 77 patients who were found to have EGFR M in tissues, 63 was detected to harbour EGFR M in peripheral blood, and high correspondence of EGFR M status in...