Abstract
A surface plasmon resonance (SPR) biosensor is employed in this study to detect Escherichiacoli O157:H7. The SPR biosensor couples antibody-antigen reactions with signal generation,using Kretschmann geometry to couple light into surface plasmons. The objective of this studywas to verify the feasibility of employing an SPR biosensor to detect E. coli O157:H7 by testingthe sensitivity and specificity of the SPR biosensor assays. Tests were conducted at near real-timewith results obtained for one SPR biosensor assay within 35 min. The limit of detection forE. coli O157:H7 was determined to be 107CFU/ml. The SPR biosensor was specific to E. coliO157:H7 in pure cultures at concentrations of 107CFU/ml. In mixed cultures, the detection limitof the biosensor was 107CFU/ml for E. coli O157:H7, when the non-target bacterialconcentration was 106CFU/ml or less. Concentrations of non-target bacteria beyond 107CFU/ml caused a decrease in the magnitude of the sensor response. The results of this studydemonstrate the potential for employing the SPR biosensor to detect E. coli O157:H7 in nearreal-time. The SPR biosensor shows potential as a tool to assist the food industry in pathogenmonitoring that is quick, sensitive, and specific. Due to its portability, it can potentially beincorporated into a HACCP protocol in the food industry.
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