Detection of Colistin Resistance in Gram Negative Pathogens: A One Year Cross-sectional Study in a Tertiary Care Centre in Northeast India

Abstract

International Journal of Medicine and Public Health,2022,12,4,175-179.DOI:10.5530/ijmedph.2022.4.32Published:January 2023Type:Original ArticleDetection of Colistin Resistance in Gram Negative Pathogens: A One Year Cross-sectional Study in a Tertiary Care Centre in Northeast IndiaDeepayan Biswas, Wihiwot Valarie Lyngdoh, Sheryl Lanong, Clarissa Jane Lyngdoh, Prithwis Bhattacharyya, and Nari Mary Lyngdoh Deepayan Biswas1, Wihiwot Valarie Lyngdoh2, Sheryl Lanong2, Clarissa Jane Lyngdoh2, Prithwis Bhattacharya3, Nari Mary Lyngdoh3 1Department of Microbiology, NEIGRIHMS, Mawdiangdiang, Shillong, Meghalaya, INDIA 2Department of Microbiology, NEIGRIHMS, Mawdiangdiang, Shillong, Meghalaya, INDIA. 3Department of Anesthesiology and Critical Care, NEIGRIHMS, Mawdiangdiang, Shillong, Meghalaya, INDIA. Abstract:Background: Indiscriminate use of colistin for gram-negative infections has led to increase incidence of colistin resistance. The problem of nosocomial infections especially caused by multi-drug-resistant gram-negative bacteria (MDR-GNB), particularly Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae is a matter of great concern. This study was conducted to find out the prevalence of colistin resistant gram-negative isolates from patients attending outdoor patient department (OPD), those admitted in wards and Intensive care unit from a Tertiary care centre in North-East India. Materials and Methods: Clinical samples obtained were processed using standard microbiological methods. The gram-negative isolates showing colistin resistance by Kirby- Bauer’s disc diffusion method were included and further subjected for MIC (minimum inhibitory concentration) testing by VITEK-2 system followed by confirmation by Broth microdilution method. Results: Colistin resistance was observed in 26 isolates out of 1040 gram-negative isolates using Broth microdilution method. The MIC values varied from 8 to ≥ 32 μg/ml. Majority of them belong to Pseudomonas species followed by Acinetobacter species and were highly resistant to ß-lactams, aminoglycosides, fluoroquinolones. Conclusion: This study highlights an increasing trend of colistin resistance amongst multidrug resistant (MDR) gram-negative isolates warranting routine screening for colistin resistance to guide appropriate therapy for future use. Keywords:Broth microdilution, Colistin, Disc diffusion, Multidrug resistant organisms, VITEK-2View:PDF (346.91 KB)