Detection of CD4+ , CD8+ and NK in peripheral Mood of patients with colorectal cancer and its relationship with micrometastasis in regional lymph nodes

Abstract

Objective To detect the positive rate of CK20 mRNA in regional lymph nodes and CD4+ ,CD8+ and NK cells in peripheral blood of colorectal cancer patients, and intestigate their relation-ship. Methods The positive rate of CD4+, CD8+ and NK cells in peripheral blood of 21 patients with colorectal cancer was detected by flow cytometry. The positive rate of CK20 mRNA in lymph nodes was tested by real time-polymerase chain reaction. The lymph node metastasis was examined pathologically. Re-suits For the opitive micrometastasis in regional lymph nodes patients,it was 16 nodes(6% ,16/281 ) by conventional pathological and 140 nodes (50%. 140/281 ) by real time-polymerase chain reaction, accord-ing to the opitive micrometastasis in regional lymph nodes restage the Dukes stage ,before the operation,the opitive rate of CD4+ , CD4+/CD8+ of patients in Dukes A and B were higher then Dukes C (P <0.05) ; and CD8+ in Dukes A patients were lower then Dukes C ( P < 0.05 ) ; and NK in Dukes B patients were higher then Dukes C ( P < 0. 05 ). The new Dukes stage was significantly correlated with CD4+ ( r = -0.497,P <0.01) ;CD4+/CD8+ (r = -0.714,P <0.01) ;CD8+ (r =0. 945,P <0.01 ). Conclusion For detecting the micrometastasis of lymph nodes, the method of realtime-pelymerase chain reaction were better than anventional pathological significantly, and the opitive micrometastasis in lymph nodes were cor-related significantly with the low immunity function of colorectal cancer patients. Key words: Colorectal carcinoma; Lymph node ; Micrometastasis ; Cytokeratin 20