Abstract
Candida albicans is a commensal fungus that is responsible for a lot of nosocomial infections in immunocompromised people. Cell culture is currently the predominant method for diagnosing candidiasis, but it is time consuming. In this study, we developed a rapid screen procedure by devising a method for detecting C. albicans with the use of electrochemical sensors. Through this experiment, we propose a method for the detection of C. albicans in the system through the use of personal glucose meters. The hemicellulase was used to break down the cell wall of C. albicans to glucose and oligo, which can be detected by a glucose meter. The spiked samples were prepared suspending C. albicans in urine and serum, demonstrating the feasibility of the developed method in a real situation.
Highlights
Published: 8 February 2021Candida are a species of fungi that can showcase polymorphism
We looked to estimate the level of Candida infection present in the system using personal glucose meters as the measuring device in order to act as early detection systems to increase the efficiency of the current diagnostic procedure and to help the prevention of Candidiasis
Mechanism of into thethe sample, thethe cellcell walls walls of albicans will be broken down into oligo and glucose, which can be detected by a com-gluof C. albicans will be broken down into oligo and glucose, which can be detected by a commercial mercial glucose meter
Summary
Candida are a species of fungi that can showcase polymorphism. Their ability to showcase polymorphism is one of the major factors for their pathogenesis in human hosts. Using other methods of testing involving mannan/anti-mannan immunoglobulin G, polymerase chain reaction (PCR), and β-D-glucan (BDG) assays, it is possible to diagnose Candidemia before blood cultures, and these methods have shown promising sensitivity/specificity Still, these methods are not widely investigated in blood culture-negative, deep-seated candidiasis [17]. We see that that most work is being focused on techniques and methods that can rapidly and detect candida non-invasively, and that can offer a high sensitivity and accuracy to match the current gold standard in the field, which is culturing cells taken from blood samples. We looked to estimate the level of Candida infection present in the system using personal glucose meters as the measuring device in order to act as early detection systems to increase the efficiency of the current diagnostic procedure and to help the prevention of Candidiasis.
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