Abstract
AbstractLectin cytochemistry was used to detect viral specific proteins in infected bovine turbinate cells as a means of diagnosing the presence of various virus. Six lectins having different properties were evaluated for binding to bovine respiratory syncytial virus (BRSV), bovine viral diarrhea (BVD), bovine herpesvirus-I (BHV-l), and bovine herpesvirus-II (BHV-2). BRSV, BHV-1, and BHV-2 were detected. Three lectins, Phaseolus limensis, Phaseolus vulgaris, and Glycine max could not detect any virus, Three lectins that could detect one or more virus included Vicia villosa B4, Helix pomatia, and Helix aspersa Lectins as a detection tool have a considerable level of specificity for binding to virus associated proteins. This work shows that this characteristic and others allow lectins to be a powerful means to diagnose viral infection. (The J Histotechnol 12:115, 1989).
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