Detection of aquatic streptomycetes by quantitative PCR for prediction of taste-and-odour episodes in water reservoirs

Abstract

Research Article| August 01 2012 Detection of aquatic streptomycetes by quantitative PCR for prediction of taste-and-odour episodes in water reservoirs Jeanette E. Lylloff; Jeanette E. Lylloff 1Department of Agriculture and Ecology, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark Search for other works by this author on: This Site PubMed Google Scholar Maria Hummelshøj Mogensen; Maria Hummelshøj Mogensen 1Department of Agriculture and Ecology, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark Search for other works by this author on: This Site PubMed Google Scholar Michele A. Burford; Michele A. Burford 2Australian Riverine Institute, Griffith University, Nathan, Queensland 4111, Australia Search for other works by this author on: This Site PubMed Google Scholar Louise Schlüter; Louise Schlüter 3Carbon 14 Agency, DHI Group, Agern Allé 5, DK-2970 Hørsholm, Denmark Search for other works by this author on: This Site PubMed Google Scholar Niels O. G. Jørgensen Niels O. G. Jørgensen 1Department of Agriculture and Ecology, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark E-mail: nogj@life.ku.dk Search for other works by this author on: This Site PubMed Google Scholar Journal of Water Supply: Research and Technology-Aqua (2012) 61 (5): 272–282. https://doi.org/10.2166/aqua.2012.006 Article history Received: January 17 2012 Accepted: July 13 2012 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Cite Icon Cite Permissions Search Site Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsThis Journal Search Advanced Search Citation Jeanette E. Lylloff, Maria Hummelshøj Mogensen, Michele A. Burford, Louise Schlüter, Niels O. G. Jørgensen; Detection of aquatic streptomycetes by quantitative PCR for prediction of taste-and-odour episodes in water reservoirs. Journal of Water Supply: Research and Technology-Aqua 1 August 2012; 61 (5): 272–282. doi: https://doi.org/10.2166/aqua.2012.006 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex Bacteria belonging to the Streptomyces genus are known to produce several taste-and-odour compounds (TOCs), but knowledge on the abundance of streptomycetes in drinking water reservoirs and other aquatic environments is scarce. In this study, quantitative real-time polymerase chain reaction (qPCR) was applied, for the first time, to determine densities of streptomycetes in a river, at a weir and in two reservoirs in subtropical Australia. The PCR approach was optimized with respect to (a) collection of streptomycetes in water, (b) extraction of DNA, and (c) a procedure to correct for inhibition of PCR amplification by natural substances in the water. Mean densities of Streptomyces cells at the study sites varied from 225 to 45,650 cells L−1. The highest density occurred in bottom water (8.5 m deep) of one of the reservoirs, while densities in the Brisbane River varied between 260 and 7,950 cells L−1. At the weir site, seasonal variation in abundance in winter and spring in surface water (mean densities of 430–13,550 cells L−1) did not correlate with total bacterial abundance (0.9–3.5 × 109 cells L−1). The qPCR approach shows that quantitation of streptomycetes in fresh water can be successfully achieved and may prove valuable in predicting TOC episodes in aquatic systems used for drinking water supplies. drinking water reservoirs, geosmin, MIB, quantitative PCR, Streptomyces This content is only available as a PDF. © IWA Publishing 2012 You do not currently have access to this content.