Abstract
Ambarawa Regency is one of the endemic sub-districts since 2017 which always accounts for the highest DHF cases in Semarang Regency. Molecular detection of Aedes aegypti resistance towards sipermetrin using Polymerase Chain Reaction (PCR) techniques. The study was conducted to see mutations in the Aedes aegypti VGSC gene.
 This research was a pure experimental study. The Aedes aegypti samples examined were 10 in every village taken from 3 endemic DHF villages with high fogging intensity in Ambarawa. The research used random sampling techniques taken with ovitrap which was first installed during the month of August. Resistance detection test using the PCR method were conducted at the Banjarnegara Research and Development Center in September. Data were analyzed descriptively to illustrate the results of the study.
 The results showed that in Tambakboyo Village, 2 samples were susceptible (V / V), 7 samples were detected as homozygous (G / G) resistant, 1 sample was detected as heterozygous (V / G) resistant; in Kupang District 5 samples were detected as being homozygous resistant (G / G) and 5 samples were detected as heterozygous resistant (V / G); and in Panjang Village 1 susceptible sample (V / V), 8 samples were detected as homozygous resistant (G / G), 1 sample was detected as heterozygous resistant (V / G).
 Based on the results of mutation studies had been found in the VGSC gene in codon V1016G. The proper implementation of management, selection and rotation of insecticides is expected to reduce the risk of resistance in the Aedes aegypti mosquito population.
Highlights
Dengue Hemorrhagic Fever (DHF) is a disease caused by Dengue virus which is classified as Arthropod-Borne Virus, genus Flavivirus, and family Flaviviridae
Resistance detection of by looking at mutations in the gen voltage gated sodium channel (VGSC) in the world occurs in populations of Aedes aegypti in Panama with the discovery of mutations for the first time in America in codons V1016G and I1011M (Murcia et al, 2019), and in Taiwan which detected mutations in the V1016G codon (28.03%) ), S989P (17.83%), F1534C (21.97%), and D1763Y (66.69%) (Chung, 2019)
Similar mutations were found in Indonesia in samples of Aedes aegypti originating from Palembang in studies showing the results of the gen V1016G with a DNA target of 82 bp (Ghiffari et al, 2013)
Summary
Dengue Hemorrhagic Fever (DHF) is a disease caused by Dengue virus which is classified as Arthropod-Borne Virus, genus Flavivirus, and family Flaviviridae. Ter in Semarang Regency in 2015-2017 show there are three public health centers (Puskesmas) with the highest number of cases, namely Ambarawa Public Health Center 267 cases and 2 cases of death, Pringapus Public Health Center 235 cases and 1 case of death, and Bergas Public Health Center is 216 and 3 cases of death (Semarang Regency Health Office, 2017). Fogging was carried out by the Semarang Regency Health Office including 9 times in Kupang Village, 8 times in Panjang Village, and 5 times in Tambakboyo Village using 15 UL zeta trademark insecticide which contains the main active ingredient zeta- cypermethrin 15 g/l (Puskesmas Ambarawa, 2019). Ambarawa Sub-district is one of the endemic sub-districts since 2017 which always contributes the highest DHF cases (Health Office in Semarang Regency, 2017)
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