Abstract
To detect an adult Schistosoma japonicum circulating antigen, we produced a monoclonal antibody (MAb), termed 1-7B, which recognized a gut-associated excretory–secretory antigen. We characterized the antigen on a two-dimensional gel electrophoretic pattern (2-D pattern) of proteins extracted from S. japonicum adult worms. The antigen formed one vertically elongated spot, with an apparent molecular weight of 60–200 kDa and an isoelectric point of<4. Since the spot was seen on a 2-D pattern of S. japonicum-infected rabbit serum but not on that of normal rabbit serum, it was ascertained that the antigen was a circulating antigen (CA). A polyclonal rabbit antiserum against the spot was prepared and used for immunoblotting analysis with S. japonicum, S. mansoni, Paragonimus miyazakii, Spirometra erinaceieuropaei and Anisakis sp. The antigen was detected only in an extract of S. japonicum. From these results, the antigen is considered to differ from the CAs reported by previous authors, because of its molecular weight, isoelectric point and specificity.
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