Abstract
The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T.gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T.gondii in fresh semen samples using invitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T.gondii was confirmed by invitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T.gondii DNA sequences stored in the GenBank. It has been shown that T.gondii tachyzoites may be isolated invitro from fresh semen samples of clinically healthy dogs seropositive for T.gondii.
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