Abstract
Background and Objectives:Helicobacter pylori (H. pylori) infection is cause of several gastrointestinal diseases in humans. Virulence genes of H. pylori are associated with severity of disease and vary geographically. The aim of present study was to detect H. pylori in formalin-fixed paraffin-embedded (FFPE) tissues and further investigate prevalence of babA2, cagA, iceA1, iceA2, vacA s1/s2 and vacA m1/m2 genotypes in H. pylori from gastric cancer (GC) and gastric ulcer (GU) patients’ biopsy samples.Methods:We used FFPE tissues of 35 GC and 10 GU patients’ biopsy samples. Using Polymerase Chain Reaction (PCR), detection of H. pylori strain was performed by using specific primers targeting 16S rRNA and ureC encodes for phosphoglucosamine mutase genes. We have identified different virulence genes of H. pylori by PCR.Results:Of all the 45 samples tested, 20 GC and all 10 GU samples were positive for identification of H. pylori using specific genes (16S rRNA and ureC). The prevalence of babA2(100%) was significantly higher in GC as compared to GU (40%) samples. The rate of virulence genes vacAs1 was higher in both GU 8 (80%) and GC (100%).Conclusions:Our study finds that vacAs1am1 and babA2 are most prominent genotypes and may play role in development of Gastric cancer.
Highlights
Helicobacter pylori (H. pylori) is a pathogenic bacterium that inhabits gastric mucosa of humans and cause several gastrointestinal diseases including gastric cancer (GC)
Among 45 formalin-fixed paraffin-embedded (FFPE) gastric tissues, 20 (57.2%) GC and 10 (100%) gastric ulcer (GU) biopsy samples were positive for H. pylori using 16S rRNA (Fig. 1a and b) and glmM genes
We examined six different H. pylori virulence genes in gastric biopsy samples
Summary
Helicobacter pylori (H. pylori) is a pathogenic bacterium that inhabits gastric mucosa of humans and cause several gastrointestinal diseases including gastric cancer (GC). Three different bab alleles have been reported where only the babA2 is functional for binding activity.[4] Previous studies have reported prevalence of babA2 positive strain in peptic ulcer and GC but with conflicting results.[5] relationship between H. pylori genotype and disease condition may vary from one geographic region to other. The aim of present study was to detect H. pylori in formalin-fixed paraffin-embedded (FFPE) tissues and further investigate prevalence of babA2, cagA, iceA1, iceA2, vacA s1/s2 and vacA m1/m2 genotypes in H. pylori from gastric cancer (GC) and gastric ulcer (GU) patients’ biopsy samples. Conclusions: Our study finds that vacAs1am[1] and babA2 are most prominent genotypes and may play role in development of Gastric cancer
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