Detection and characterization of a bacteriocin, putadicin T01, produced by Pseudomonas putida isolated from hot spring water.

Abstract

Pseudomonas strains isolated from hot spring water were tested for bacteriocin-like substance (BLS) production using a target panel of closely related microorganisms and other Gram-positive and Gram-negative bacteria. Molecular identification was carried out through specific PCR and 16S RNA sequence analysis. Isolates were identified as Brevundimonas diminuta and Pseudomonas putida, the latter exhibited antimicrobial activity. Pseudomonas putida strains produce an inhibitory substance against other Pseudomonas strains and other species including food-borne pathogens. The BLS was sensitive to the proteolytic action of proteinase K, pronase E and trypsin but resistant to α-amylase, RNase and lipase C, reflecting its proteinaceous nature. The BLS was stable at 100°C and also after thermal treatment at 121°C for 15min. Additionally, it was stable within a wide range of pH (2-10). The substance from P. putida T01 strain was bactericidal to Escherichia coli. SDS-PAGE analysis of the partial purified supernatant of strain T01 revealed a BLS with an approximate molecular mass of 8kDa. Therefore, the results of this study show that P. putida strain T01 produces a BLS with a higher activity spectrum, which may find application in human medicine and in minimally processed food preservation.