Abstract
Gastroenteritis in young animals is a clinical presentation with many infectious and non- infectious aetiologies. We used next generation sequencing (NGS) to investigate the possible infectious causes of gastroenteritis in puppies from a dog kennel in Victoria, Australia. The near complete genome of a canine astrovirus was obtained from pooled faecal samples, and was found to be 94.7% identical with a canine astrovirus detected in the United Kingdom in 2012. The phylogenetic analysis of the capsid gene found similarities to those of canine astroviruses identified in Italy in 2005 and in UK and Hungary in 2012, but distant from that of a canine astrovirus previously identified in Australia in 2012. Thus, different serotypes of canine astrovirus are likely circulating in Australia. The close relationship to European astroviruses also suggested that there had been recent movements of ancestor canine astroviruses between Australia and Europe. NGS also detected other infections in the puppies including several canine papillomaviruses and a canine parvovirus (vaccine strain) as well as a very low level of campylobacter. Canine astrovirus was the probable cause of diarrhoea in these puppies, with the possible involvement of campylobacter bacteria. NGS was effective as a non-targeted method to determine the likely infectious cause of gastroenteritis.
Highlights
Canine infectious gastroenteritis is one of the common reasons for presentation to a veterinary clinic and hospitalization
When it has been used, it has detected a diverse range of viruses in dogs with diarrhoea including canine sapovirus, canine kobuvirus, canine parvovirus, canine astrovirus, canine rotavirus and canine coronavirus in dogs with acute diarrhoea[13,14]
The puppies were sick with diarrhoea/intestinal problems and Campylobacter was tentatively diagnosed at the local veterinary hospital
Summary
Canine infectious gastroenteritis is one of the common reasons for presentation to a veterinary clinic and hospitalization It can be challenging for the veterinarian to determine the actual causative agent responsible for diarrhoea due to the diverse array of pathogenic agents which could be responsible including viruses, bacteria, or protozoans[1,2]. Its application to the diagnosis of the causes of gastroenteritis in dogs has been limited to date[13,14] When it has been used, it has detected a diverse range of viruses in dogs with diarrhoea including canine sapovirus, canine kobuvirus, canine parvovirus, canine astrovirus, canine rotavirus and canine coronavirus in dogs with acute diarrhoea[13,14]. The aim was to determine if generation sequencing could be used to identify the possible cause(s) of the gastroenteritis
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
