Abstract
A cutting-edge high-performance thin layer chromatography (HPTLC) approach designed for simultaneous measurement of tablets containing drugs like lamivudine, nevirapine, and zidovudine. Chromatograms were performed employing chloroform and methanol (8:2 v/v) on pre-coated silica gel aluminum thin-layer chromatography (TLC) plates. The data was quantified using the optical density absorbance mode at 271 nm. The Rf values for lamivudine, zidovudine, and nevirapine were 0.140, 0.523, and 0.678, respectively. Linearity was found for lamivudine, zidovudine and nevirapine at concentrations of 150 to 900 ng band-1, 300 to 1800 and 200 to 1200 ng band-1, respectively. Lamivudine had a lower limit of detection (LLoD) of 27.36 ng band-1 and a lower limit of quantitation (LLoQ) of 82.91 ng band-1, whereas zidovudine had LoQs of 64.13 ng band-1 and 194.34 ng band-1. For nevirapine, the values were 35.52 and 107.64 ng band-1, respectively. Lamivudine had a lower LoD of 27.36 ng band-1 and a lower LoQ of 82.91 ng band-1, whereas zidovudine had LoQs of 64.13 and 194.34 ng band-1. For nevirapine, the values were 35.52 and 107.64 ng band-1, respectively. The recovery, specificity, and accuracy of this device have been demonstrated. This new formulation evaluated market tablet formulations of the above drugs (Duovir, Cipla Ltd.). The developed method demonstrated the ability to simultaneously identify these drugs from quantitative data without interference.
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