Abstract
AbstractIcariin molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization. Prior to the polymerization, computer simulation was performed to sketchily choose the suitable functional monomer and the corresponding polymerization solvent. The optimized synthesis parameters, including the functional monomer acrylamide, the mixture of methanol and acetonitrile (V:V = 3:1) as the polymerization solvent, and the reaction molar ratio (1:6:80) of template molecule, functional monomer and cross-linker, were respectively obtained by single factor analysis and orthogonal design methods. The results of the adsorption experiments showed that the resultant MIPs exhibited good adsorption and recognition abilities to icariin. Scatchard analysis illustrated that the homogeneous binding sites only for icariin molecules were formed in the prepared MIPs.
Highlights
Molecular imprinting technique (MIT) is a new technology developed through simulating the interaction between antigen and antibody in vivo which forms selective sites in a polymer matrix with the memory of the template molecule due to shape recognition, hydrogen bonding and hydrophobic interactions (Cirillo, Curcio, Parisi, Puoci, & Iemma, 2011; Zeng, Wang, Liu, Kong, & Nie, 2012)
The experimental results showed that the prepared molecularly imprinted polymers (MIPs) had high affinity and selectivity to the natural drug icariin, and they were of great application potential to separate and purify icariin from the Chinese medicinal herb epimedium
40.0 mg of MIPs or non-molecularly imprinted polymers (NIPs) were added into conical flasks which contained 25 mL of 0.0140 mg mL−1 icariin solution, the flasks were stirred at room temperature, and part of the solution were taken out and centrifuged at the defined time
Summary
Molecular imprinting technique (MIT) is a new technology developed through simulating the interaction between antigen and antibody in vivo which forms selective sites in a polymer matrix with the memory of the template molecule due to shape recognition, hydrogen bonding and hydrophobic interactions (Cirillo, Curcio, Parisi, Puoci, & Iemma, 2011; Zeng, Wang, Liu, Kong, & Nie, 2012). Epimedium is one of the Chinese traditional herbal medicines, whose major active ingredient icariin has a wide range of pharmacological activities including regulating cardiovascular, circulatory, genital, and bone marrow systems, stimulating neurite growth, possessing estrogenic function, enhancing bone healing, and treating osteoporosis. The extraction of trace amounts of icariin from epimedium with traditional methods is tedious and a bit inefficient. The experimental results showed that the prepared MIPs had high affinity and selectivity to the natural drug icariin, and they were of great application potential to separate and purify icariin from the Chinese medicinal herb epimedium. Imprinted polymers (MIPs) can be synthesized using MIT by co-polymerizing the functional monomers and the cross-linkers in the presence of template molecules in solution. The experimental results showed that MIPs prepared in this work have good selectivity and high affinity to icariin
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