Abstract
Surface-active artificial enzymes (SAEs) are designed and constructed by a general and novel strategy. These SAEs can simultaneously stabilize Pickering emulsions and catalyze biphasic biotransformation with superior enzymatic stability and good re-usability; for example, for the interfacial conversion of hydrophobic p-nitrophenyl butyrate into yellow water-soluble p-nitrophenolate catalyzed by esterase-mimic SAE.
Highlights
Enzymes represent as effective and sometimes preferable catalysts for a wide range of important organic reactions on account of their high chemo, regio- and enantioselectivity.[1]in many cases, substrates of interest are sparingly soluble in water whereas enzymes are typically active in the water phase
By embedding the beads in resin and cutting them into microtomed slices, esteraselike activity (ESAE) particles were clearly seen only at the periphery of the beads with high-resolution SEM (Figure 2g). These results provided direct evidence for the pronounced interfacial activity of the obtained ESAE particles in Pickering emulsions
The catalytic activity of the obtained ESAE particles at oil-water interfaces was studied with the hydrolysis of oil-soluble p-nitrophenyl butyrate (PNPB) using dodecane as the organic medium (Figure 3a)
Summary
Enzymes represent as effective and sometimes preferable catalysts for a wide range of important organic reactions on account of their high chemo-, regio- and enantioselectivity.[1]in many cases, substrates of interest are sparingly soluble in water whereas enzymes are typically active in the water phase. Surface-active artificial enzyme (SAE) particles were designed and constructed as a new generation of interfacial biocatalysts for oil-in-water (o/w) Pickering emulsions and their ability for high-performance biphasic biocatalysis is demonstrated. By positionally locating the wettability-tuning groups and catalytic locus over and within porous colloidal particles, SAE capable of simultaneously stabilizing Pickering emulsions and catalyzing biphasic reactions could be obtained.
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